1) Wang C, Swerdloff RS. Limitations of semen analysis as a test of male fertility and anticipated needs from newer tests. Fertil Steril. 2014;102:1502-7.
2) Agarwal A, Majzoub A, Baskaran S, et al. Sperm DNA fragmentation:A new guideline for Clinicians. World J Mens Health. 2020;38:412-71.
3) Zini A. Are sperm chromatin and DNA defects relevant in the clinic? Syst Biol Reprod Med. 2011;57:78-85.
4) Zhao J, Zhang Q, Wang Y, et al. Whether sperm deoxyribonucleic acid fragmentation has an effect on pregnancy and miscarriage after in vitro fertilization/intracytoplasmic sperm injection:a systematic review and meta-analysis. Fertil Steril. 2014;102:998-1005.
5) Borges E Jr, Zanetti BF, Setti AS, et al. Sperm DNA fragmentation is correlated with poor embryo development, lower implantation rate, and higher miscarriage rate in reproductive cycles of non-male factor infertility. Fertil Steril. 2019;112:483-90.
6) Hervas I, Pacheco A, Gil Julia M, et al. Sperm deoxyribonucleic acid fragmentation(by terminal deoxynucleotidyl transferase biotin dUTP nick end labeling assay)does not impair reproductive success measured as cumulative live birth rates per donor metaphase II oocyte used. Fertil Steril. 2022;118:79-89.
7) Lira Neto FT, Roque M, Esteves SC. Effect of varicocelectomy on sperm deoxyribonucleic acid fragmentation rates in infertile men with clinical varicocele:a systematic review and meta-analysis. Fertil Steril. 2021;116:696-712.
8) Smit M, Romijn JC, Wildhagen MF, et al. Decreased sperm DNA fragmentation after surgical varicocelectomy is associated with increased pregnancy rate. J Urol. 2013;189:S146-50.
9) Agarwal A, Mulgund A, Alshahrani S, et al. Reactive oxygen species and sperm DNA damage in infertile men presenting with low level leukocytospermia. Reprod Biol Endocrinol. 2014;12:126.
10) Gallegos G, Ramos B, Santiso R, et al. Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma. Fertil Steril. 2008;90:328-34.
11) Sanchez-Martin P, Sanchez-Martin F, Gonzalez-Martinez M, et al. Increased pregnancy after reduced male abstinence. Syst Biol Reprod Med. 2013;59:256-60.
12) de Ligny W, Smits RM, Mackenzie-Proctor R, et al. Antioxidants for male subfertility. Cochrane Database Syst Rev. 2022;5:Cd007411.
13) Agarwal A, Wang SM. Clinical relevance of oxidation-reduction potential in the evaluation of male infertility. Urology. 2017;104:84-9.
14) Yamasaki K, Uchida M, Watanabe N, et al. Effects of antioxidant co-supplementation therapy on spermatogenesis dysfunction in relation to the basal oxidation-reduction potential levels in spermatozoa:A pilot study. Reprod Med Biol. 2022;21:e12450.
15) Simon L, Zini A, Dyachenko A, et al. A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl. 2017;19:80-90.
16) Zhao J, Zhang Q, Wang Y, et al. Whether sperm deoxyribonucleic acid fragmentation has an effect on pregnancy and miscarriage after in vitro fertilization/intracytoplasmic sperm injection:a systematic review and meta-analysis. Fertil Steril. 2014;102:998-1005.e1008.
17) Quinn MM, Jalalian L, Ribeiro S, et al. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod. 2018;33:1388-93.
18) Quinn MM, Ribeiro S, Juarez-Hernandez F, et al. Microfluidic preparation of spermatozoa for ICSI produces similar embryo quality to density-gradient centrifugation:a pragmatic, randomized controlled trial. Hum Reprod. 2022;37:1406-13.
19) Franco JG Jr, Baruffi RL, Mauri AL, et al. Significance of large nuclear vacuoles in human spermatozoa:implications for ICSI. Reprod Biomed Online. 2008;17:42-5.
20) Parmegiani L, Cognigni GE, Bernardi S, et al."Physiologic ICSI":hyaluronic acid(HA)favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil Steril. 2010;93:598-604.
21) Miller D, Pavitt S, Sharma V, et al. Physiological, hyaluronan-selected intracytoplasmic sperm injection for infertility treatment(HABSelect):a parallel, two-group, randomised trial. Lancet. 2019;393:416-22.
22) West R, Coomarasamy A, Frew L, et al. Sperm selection with hyaluronic acid improved live birth outcomes among older couples and was connected to sperm DNA quality, potentially affecting all treatment outcomes. Hum Reprod. 2022;37:1106-25.
23) Esteves SC, Roque M, Bradley CK, et al. Reproductive outcomes of testicular versus ejaculated sperm for intracytoplasmic sperm injection among men with high levels of DNA fragmentation in semen:systematic review and meta-analysis. Fertil Steril. 2017;108:456-67.e451.
24) Mehta A, Esteves SC, Schlegel PN, et al. Use of testicular sperm in nonazoospermic males. Fertil Steril. 2018;109:981-7